Fine Mapping and Candidate Gene Analysis of the Gv1 Locus Controlling Green-Peel Color in Eggplant (Solanum melongena L.)

Peel color is one of the most important appearance qualities eggplant. The main pigment in peel green-fruited eggplant chlorophyll, while white-fruited a mutant chlorophyll biosynthesis. A dominant gene Gv controls biosynthesis peel, but none its genes have been mapped. In this study, white-peel inbred line 19141, green-peel 19143, and their F2 progeny with 3:1 segregation ratio plants to plants, were used map Gv1 locus by whole genome re-sequencing combined bulked segregant analysis (BSA). was mapped region spanning 7.66 Mb on chromosome 8, which narrowed down 173.2 kb interval screening recombinant InDel SNP markers. Sixteen candidate annotated above closely associated region. With aid RNA-Seq data, it speculated that SmAPPR2-like for Gv1. results cloning sequencing showed there might three types mutation (large deletion, frameshift variant premature stop codon) accessions, these, deletion mutation, such as common. Based sequence difference SmAPPR2-like, molecular markers developed distinguish white accessions natural populations, other genetic variations leading elucidated. present study not only provided reliable MAS (marker-assisted selection) breeding color, also paved way understanding mechanism fruit.